Novel Heteronuclear Magnetization Transfer Experiments involving SARS-CoV2 Derived RNA's
Physics
In this work, a novel experiment using selective longitudinal Cross Polarization is proposed and demonstrated that enhances the sensitivity and resolution of homonuclear NOESY correlations by targeting selected 1H–15N spin pairs.The enhanced signal sensitivity (~ 2-5) as well as access to both 15N–1H and 1H–1H NOESY dimensions can greatly facilitate RNA assignments and secondary structure determinations, as demonstrated with the analysis of genome fragments derived from the SARS-CoV-2 virus. More details at: https://doi.org/10.1021/jacs.1c01914
Ref: J. Am. Chem. Soc. 2021, 143, 13, 4942–4948; Jihyun Kim, Mihajlo Novakovic, Sundaresan Jayanthi, Adonis Lupulescu, Eriks Kupce, J Tassilo Grün, Klara Mertinkus, Andreas Oxenfarth, Christian Richter, Robbin Schnieders, Julia Wirmer-Bartoschek, Harald Schwalbe, Lucio Frydman.